Detailed Abstract
[E-poster - Biliary & Pancreas]
[EP 184] THE IMPORTANCE OF LABORATORY RESEARCH METHODS IN THE DIAGNOSIS AND TREATMENT OF BILIARY PANCREATITIS
Farukh Mahmadzoda1, Kamol Barotov1, Akbar Murodov3, Davlatmurod Sadulloev2, Abdurahmon Ashurov 1, Farukh Mahmadzoda1
Department of Surgical Diseases №1, Avicenna Tajik State Medical University, Dushanbe, Tajikistan1, Department of Surgical Diseases №1, Institute of Gastroenterology of the Republic of Tajikistan, Dushanbe, Tajikistan2, Department of Surgical Diseases №1, Scientific Center of Oncology of the Republic of Tajikistan, Dushanbe, Tajikistan3
Background : To study the importance of cytokines and lipid peroxidation in the diagnosis and treatment of biliary pancreatitis (BP).
Methods : The study is based on an analysis of the results of treatment of 52 patients with various forms of BP. The comprehensive diagnosis of patients includes the study of lipid peroxidation and the cytokine system.
Results : The level of IL-6 was increased to 304.21±12.03 pg/ml, IL-8 - to 2891.18±88.1 pg/ml. An increase in the ability of blood cells to produce TNF-α was noted, the level of which was 835.52±22.04 pg/ml, which is significantly higher than that in healthy people - 289.33±17.39 pg/ml. The level of the pro-inflammatory cytokine IL-4 was significantly lower than its value in the control group: 57.1±7.5 pg/ml versus 185.9±13.92 pg/ml. Thus, a study of the ability of blood cells to produce interleukins revealed an increase in the level of pro-inflammatory cytokines by 2-3 times in relation to the indicators of practically healthy individuals and a decrease in the level of the pro-inflammatory cytokine IL-4. The content of diene conjugates in the plasma of patients with BP was 5.40±0.25 units/ml, which significantly exceeded their level in healthy individuals - 3.02±0.08 units/ml. The average content of ketodienes in patients with biliary pancreatitis was 3.59 units/ml, and in healthy people, respectively, -1.63±0.13 units/ml. Thus, the data obtained indicate the tension of the enzymatic antioxidant defense system, which is probably compensatory in nature in response to the excessive generation of reactive oxygen species and a pronounced intensification of the processes of free radical lipid peroxidation.
Conclusions : The inflammatory process in patients with BP is accompanied by dysregulation of cytokines, immunocompetent cells and an increase in the intensity of lipid peroxidation processes with inhibition of enzymatic binding of antioxidant defense. These factors play an important role in the diagnosis and correction of BP.
Methods : The study is based on an analysis of the results of treatment of 52 patients with various forms of BP. The comprehensive diagnosis of patients includes the study of lipid peroxidation and the cytokine system.
Results : The level of IL-6 was increased to 304.21±12.03 pg/ml, IL-8 - to 2891.18±88.1 pg/ml. An increase in the ability of blood cells to produce TNF-α was noted, the level of which was 835.52±22.04 pg/ml, which is significantly higher than that in healthy people - 289.33±17.39 pg/ml. The level of the pro-inflammatory cytokine IL-4 was significantly lower than its value in the control group: 57.1±7.5 pg/ml versus 185.9±13.92 pg/ml. Thus, a study of the ability of blood cells to produce interleukins revealed an increase in the level of pro-inflammatory cytokines by 2-3 times in relation to the indicators of practically healthy individuals and a decrease in the level of the pro-inflammatory cytokine IL-4. The content of diene conjugates in the plasma of patients with BP was 5.40±0.25 units/ml, which significantly exceeded their level in healthy individuals - 3.02±0.08 units/ml. The average content of ketodienes in patients with biliary pancreatitis was 3.59 units/ml, and in healthy people, respectively, -1.63±0.13 units/ml. Thus, the data obtained indicate the tension of the enzymatic antioxidant defense system, which is probably compensatory in nature in response to the excessive generation of reactive oxygen species and a pronounced intensification of the processes of free radical lipid peroxidation.
Conclusions : The inflammatory process in patients with BP is accompanied by dysregulation of cytokines, immunocompetent cells and an increase in the intensity of lipid peroxidation processes with inhibition of enzymatic binding of antioxidant defense. These factors play an important role in the diagnosis and correction of BP.
SESSION
E-poster
E-Session 03/21 ALL DAY