Detailed Abstract
[Poster Presentation 5 - Liver (Liver Disease/Surgery)]
[LV PP 5-S4] Umbelliferone loaded Nano-Lipidic Carriers regulates mitochondrial apoptosis and PhD2 mediated hypoxia in Diethyl nitrosamine induced Hepatocellular Carcinoma
Vikas Kumar1, D Chauhan1
Department of Hepatobiliary and Pancreatic Surgery, SGSS Institute, Department of Hepatobiliary and Pancreatic Surgery, SGSS Institute
Background : Hepatocellular carcinoma (HCC) is the 3rd most common cause of mortality, world widely. Clinical and medical fraternity is aware of efficient treatment of HCC such as liver transplantation and surgical resection is viable option, but both the treatment having the limitations either due to patient condition or late detection of HCC. In this study, we scrutinized the chemo-protective effect of Umbelliferone loaded Nano-Lipidic Carriers (UF-NLCs) against diethylnitrosamine (DEN) induced hepatic carcinoma (HCC) via regulates mitochondrial apoptosis and PhD2 mediated hypoxia.
Methods : Intraperitoneal injection of DEN (200 mg/kg) was introduced for induction the HCC. The rats were received the UF-NLCs for 20 weeks. The progression of serum biomarkers, morphological evaluation and histopathological components of hepatic tissue were used to access the prophylactic effects. The inflammatory, pro-inflammatory, cancer preventive agent status, antioxidant enzymes and apoptosis mechanism were appraised to examine the possible mechanism.
Results : UF-NLCs decreased the hepatic nodules (65%) and increased the body weight (51.5%); altered the hepatic parameter like AFP (73.3%), CEA (65.6%), GGT (54.4%), AST (58.9%), ALP (58.6%), ALT (58.7%); non-hepatic parameter viz., albumin (55.3%), total protein (45.5%), BUN (52.3%), total bilirubin (45.5%), direct bilirubin (54.4%); antioxidant parameter LPO(43.3%), CAT (47.5%),SOD (48.8%), GST (53.4%), GPx (53.4%) and caspase-3 (34.5%) and caspase-7 (45.5%), respectively. UF-NLCs considerably modulated the mitochondria mediated apoptosis viz., cytochrome c (23%), VADC (34.5%), pro-caspase 9 (35.5%), Apaf-1 (35.6%), cytochorme c (35.5%); hypoxia expression such as HIF-1α (34%), FASN (45%), UCHL-1 (40.5%),SREBP-1c (42.3%), NFκBp65 (45.5%) and PHD2 (35.5%), respectively.Histopathological observation also established and supported the above result by UF and UF-NLC.
Conclusions : Furthermore, UF-NLCs proved its chemoprotective effect against DEN induced HCC via alteration of mitochondrial mediated death apoptosis, hypoxic microenvironment and anti-inflammatory pathway.
Methods : Intraperitoneal injection of DEN (200 mg/kg) was introduced for induction the HCC. The rats were received the UF-NLCs for 20 weeks. The progression of serum biomarkers, morphological evaluation and histopathological components of hepatic tissue were used to access the prophylactic effects. The inflammatory, pro-inflammatory, cancer preventive agent status, antioxidant enzymes and apoptosis mechanism were appraised to examine the possible mechanism.
Results : UF-NLCs decreased the hepatic nodules (65%) and increased the body weight (51.5%); altered the hepatic parameter like AFP (73.3%), CEA (65.6%), GGT (54.4%), AST (58.9%), ALP (58.6%), ALT (58.7%); non-hepatic parameter viz., albumin (55.3%), total protein (45.5%), BUN (52.3%), total bilirubin (45.5%), direct bilirubin (54.4%); antioxidant parameter LPO(43.3%), CAT (47.5%),SOD (48.8%), GST (53.4%), GPx (53.4%) and caspase-3 (34.5%) and caspase-7 (45.5%), respectively. UF-NLCs considerably modulated the mitochondria mediated apoptosis viz., cytochrome c (23%), VADC (34.5%), pro-caspase 9 (35.5%), Apaf-1 (35.6%), cytochorme c (35.5%); hypoxia expression such as HIF-1α (34%), FASN (45%), UCHL-1 (40.5%),SREBP-1c (42.3%), NFκBp65 (45.5%) and PHD2 (35.5%), respectively.Histopathological observation also established and supported the above result by UF and UF-NLC.
Conclusions : Furthermore, UF-NLCs proved its chemoprotective effect against DEN induced HCC via alteration of mitochondrial mediated death apoptosis, hypoxic microenvironment and anti-inflammatory pathway.
SESSION
Poster Presentation 5
Zone F 3/21/2024 2:50 PM - 3:30 PM